Thermo Fisher Scientific MUC2 Monoclonal Antibody (SPM512)
인체 MUC2 단백질을 인식하는 Mouse IgG1 단일클론 항체로, IHC(P) 실험에 적합. 열매개 항원 회복 시 10mM citrate buffer 권장. Protein A/G 정제, PBS+BSA buffer, -20°C 보관. 연구용 전용 제품.
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Thermo Fisher Scientific MUC2 Monoclonal Antibody (SPM512)
Applications and Tested Dilution
- Immunohistochemistry (Paraffin) (IHC (P)): 1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM512 |
| Immunogen | Synthetic peptide corresponding to a site on the MUC-2 glycoprotein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2148844 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with the primary antibody for 30 minutes at room temperature.
A suggested positive control is small intestine tissue.
Target Information
Secreted epithelial mucins are large macromolecules exhibiting extreme polydispersity.
Mucin 2 is the major intestinal mucin. O-glycans are attached to MUC2 in a potentially diverse arrangement, which is crucial for their interaction with endogenous and exogenous lectins.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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