Thermo Fisher Scientific USP5/IsoT Polyclonal Antibody
USP5/IsoT 단백질을 표적하는 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합. Human 반응성, 항원 친화 크로마토그래피로 정제. 액상 형태, 1 mg/mL 농도, 4°C 보관. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 375–425 of Human ubiquitin specific peptidase 5 (isopeptidase T) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It is involved in unanchored Lys-48-linked polyubiquitin disassembly and also binds linear and Lys-63 linked polyubiquitin with lower affinity. Knock-down of USP5 causes accumulation of p53/TP53 and increases its transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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