Thermo Fisher Scientific CCL3 Polyclonal Antibody
Thermo Fisher Scientific의 CCL3 Polyclonal Antibody는 마우스 CCL3/MIP-1α를 인식하는 염소 유래 IgG 항체입니다. WB, IHC, ICC, ELISA, Neutralization 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며, 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–15 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL |
| ELISA | 0.1–0.4 µg/mL (Detection), 0.2–0.8 µg/mL (Capture) |
| Neutralization (Neu) | 0.05–0.2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant mouse CCL3/MIP-1α (Ala24–Ala92) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609518 |
Product Specific Information
- In sandwich ELISAs, less than 0.03% cross-reactivity with recombinant human CCL3, mouse CCL9/10, and mouse CCL4, and less than 0.4% cross-reactivity with human CCL7.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level <0.10 EU per 1 µg of antibody (LAL method).
Target Information
MIP-1α and MIP-1β are CC chemokines involved in host defense against bacterial, viral, parasitic, and fungal infections. They regulate the trafficking and activation of inflammatory cells such as macrophages, lymphocytes, and NK cells.
MIP-1α selectively attracts CD8+ lymphocytes, while MIP-1β attracts CD4+ lymphocytes. Both are potent chemoattractants for B cells, eosinophils, and dendritic cells, and are active on human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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