
Thermo Fisher Scientific IL-17A Polyclonal Antibody
Mouse IL-17A에 특이적인 goat polyclonal antibody로, Western blot, Flow cytometry, Neutralization 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS/trehalose buffer에 동결건조 상태로 제공. 높은 특이성과 낮은 교차반응률을 보임.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | 2.5 µg per million cells | — |
| Neutralization (Neu) | 0.05–0.25 µg/mL | — |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant mouse IL-17 (Thr22–Ala158) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607353 |
Product Specific Information
- In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse IL-17F is observed.
- Less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, and rmIL-17E.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of antibody (LAL method).
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in conditions such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A is a 32 kDa disulfide-linked homodimer (136 amino acids) belonging to the IL-17 family (IL-17A–F) and signals through IL-17R/CDw217.
It enhances ICAM-1 expression in fibroblasts and stimulates various cells to secrete IL-6, IL-8, G-CSF, and PGE2.
IL-17A regulates NF-kappa B and MAP kinase activities, stimulates IL-6 and COX-2 expression, and increases nitric oxide production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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