Thermo Fisher Scientific C17orf96 Recombinant Rabbit Monoclonal Antibody (5H7L8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunocytochemistry (ICC/IF)

1:100

-

ChIP assay (ChIP)

2.5µg/10^6 cells

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

5H7L8

Immunogen

Peptides corresponding to human C17orf96 [aa130-aa148, aa360-aa379] if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:C17orf96,uniProtId:A6NHQ4-1,ncbiNodeId:9606,antigenRange:130-148|360-379,antigenLength:379,antigenImageFileName:703052_C17orf96_A6NHQ4-1_Rabbit.svg,antigenImageFileNamePDP:703052_C17orf96_A6NHQ4-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809223

Product Specific Information

This antibody is predicted to react with Monkey, Pig, Cat, Dog.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Elongin BC and Polycomb repressive complex 2-associated protein (EPOP) also know as C17ORF96 is a scaffold protein that serves as a bridging partner between the PRC2/EED-EZH2 complex and the elongin BC complex. It is required to fine tune the transcriptional status of polycomb group (PcG) target genes in embryonic stem cells (ESCs). C17ORF96 also plays a key role in genomic regions that display both active and repressive chromatin properties in pluripotent stem cells by sustaining low level expression of PcG target genes. It acts by recruiting the elongin BC complex, thereby restricting excessive activity of the PRC2/EED-EZH2 complex. C17ORF96 also has interacts with USP7 and promotes deubiquitination of H2B at promoter sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.