Thermo Fisher Scientific Calprotectin/MRP14/S100A9/Calgranulin B Monoclonal Antibody (47-8D3 (DF3973))
Calprotectin/MRP14/S100A9 단백질을 특이적으로 인식하는 단클론 항체로, 인간 및 랫트 시료에 적용 가능. IHC, ICC/IF, Flow Cytometry에 사용 가능. 단백질 A/G 정제, 액상 형태, 4°C 보관. 염증 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 47-8D3 (DF3973) |
| Immunogen | Human peripheral blood monocyte components |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises about 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages, and ileal tissue eosinophils.
Peripheral blood monocytes carry the antigen extra- and intracellularly, while neutrophils only intracellularly.
The S100A8/A9 complex exhibits antibacterial, antifungal, immunomodulating, and antiproliferative effects and acts as a potent chemotactic factor for neutrophils.
Plasma concentrations are elevated in diseases associated with increased neutrophil activity, such as inflammatory bowel disease.
Calprotectin is also detectable in feces and other body fluids (plasma, saliva, gingival crevicular fluid, stools, synovial fluid) during infections and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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