Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), NovaFluor Red 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 561
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 421 / 650 / 711
• eFluor 450 / 660
• FITC
• Functional Grade
• NovaFluor Red 700
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright 436 / 645
• Request custom conjugation available

Product Specific Information

The A7R34 monoclonal antibody reacts with mouse CD127, the high-affinity alpha subunit of the IL-7 receptor.

• Expressed by immature B cells in bone marrow and specific thymocyte subsets (CD4⁻CD8⁻, CD4⁺, CD8⁺).
• Mature T cells express CD127 at low levels.
• A7R34 inhibits IL-7 binding and is useful for in vivo/in vitro studies of IL-7 roles in T/B cell development and activation.
• Blocks binding of SB/199 antibody recognizing mouse CD127.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse thymocytes and splenocytes at ≤1.0 µg/test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test. Optimize concentration empirically.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before adding to cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures engineered for optimized fluorescent signatures minimizing spillover and spread.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

Target Information

CD127 (Interleukin-7 receptor alpha chain) is a glycoprotein involved in lymphopoiesis regulation.

• Forms a functional heterodimer with the gamma(c) chain.
• Promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4⁺/CD8⁺ T cells.
• Dysfunction is associated with severe combined immunodeficiency (SCID) variants.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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