Thermo Fisher Scientific CD138 (Syndecan-1) Monoclonal Antibody (DL-101), PE-Cyanine7, eBioscience

Applications and Tested Dilution

Flow Cytometry (Flow)

• Recommended usage: 5 µL (0.5 µg)/test
• Tested on U266 cell line
• View 7 publications

Product Specifications

Available formats:

• Unconjugated
• APC
• FITC
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The DL-101 monoclonal antibody reacts with human CD138 (syndecan-1), a transmembrane proteoglycan containing chondroitin sulfate and heparin sulfate chains that mediate binding to extracellular matrix components.
CD138 is absent in mature B cells but expressed in pre-B and plasma cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated; validated with U266 cells
• Usage: 5 µL (0.5 µg) per 100 µL cell sample (10⁵–10⁸ cells/test)
• Light Sensitivity: Protect from light; PE-Cy7 is sensitive to photo-induced oxidation
• Fixation:
  • Use IC Fixation Buffer (#00-8222-49)
  • Or 1-step Fix/Lyse Solution (#00-5333-54)
  • Store fixed samples up to 3 days at 4°C in the dark
• Excitation/Emission: 488–561 nm / 775 nm
• Laser Compatibility: Blue, Green, Yellow-Green lasers
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD138 (Syndecan-1) is a transmembrane proteoglycan that binds cytokines and extracellular matrix components, influencing development and tissue repair.
It is expressed in differentiating keratinocytes, plasma cells, fibroblasts, vascular smooth muscle, and endothelial cells.
CD138 expression is often correlated with cancer progression, and soluble sCD138 levels can serve as a prognostic biomarker.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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