Thermo Fisher Scientific p16INK4a Recombinant Mouse Monoclonal Antibody (BC42)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• For immunocytochemistry, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE cell sections.

Target Information

This gene generates several transcript variants differing in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase.
The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a structurally unrelated protein.
The ARF product stabilizes the tumor suppressor protein p53 by interacting with and sequestering the E3 ubiquitin-protein ligase MDM2, which is responsible for p53 degradation.
Despite structural and functional differences, both the CDK inhibitor isoforms and the ARF product share a common role in cell cycle G1 control through regulation of CDK4 and p53.
This gene is frequently mutated or deleted in various tumors and is recognized as an important tumor suppressor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.