Thermo Fisher Scientific GRP94 Polyclonal Antibody
GRP94 단백질을 인식하는 Thermo Fisher Scientific의 Goat Polyclonal Antibody. Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. Human 및 Mouse 반응성. 고순도 액상 항체로 안정적인 신호 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.3 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (KEGVKFDESEKTKE) corresponding to internal amino acids of HSP90B1 (aa 593–606) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10981529 |
Product Specific Information
This antibody is predicted to react with bovine, canine, human, porcine, and zebrafish based on sequence homology.
Tested in Peptide ELISA with an antibody detection limit dilution of 1:32,000.
Target Information
GRP94 is an 803 amino acid protein belonging to the heat shock protein 90 family. It functions as a molecular chaperone involved in processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulate CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC).
It plays a central role in innate and acquired immunity, dendritic cell maturation and chemotaxis, antibody production, and cross-priming. GRP94 is also a potential marker in breast cancer and participates in peptide loading of MHC class I molecules.
Expression of GRP94 suppresses A23187-induced apoptosis and stabilizes calcium homeostasis. It is expressed in melanoma, liver metastases of colon carcinoma, and human gastric carcinoma BGC-823 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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