Thermo Fisher Scientific RIP3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

20 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide corresponding to 14 amino acids near the carboxy terminus of murine RIP3. The immunogen is located within the last 50 amino acids of RIP3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RIP3,uniProtId:Q9QZL0-1,ncbiNodeId:10090,antigenRange:486,antigenLength:486,antigenImageFileName:PA5-19956_RIP3_Q9QZL0-1_Rabbit.svg,antigenImageFileNamePDP:PA5-19956_RIP3_Q9QZL0-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154832

Product Specific Information

Suggested positive controls are Rat Thymus Lysate, 3T3/NIH Cell Lysate, or C2C12 Cell Lysate.

PA5-19956 can be used with blocking peptide PEP-0081.

Target Information

RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, which contains a C-terminal domain unique from other RIP family members. RIPK3 is predominantly localized to the cytoplasm, and specifically, are colocalized in the mitochondrion which can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. RIPK3 is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor and caspase-8 prevents RIPK3-dependent necrosis without inducing apoptosis by functioning in a proteolytically active complex with CFLAR and that this complex is required for the protective function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.