Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Alexa Fluor 700, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Alexa Fluor 700, eBioscience

Applications

• Immunohistochemistry (IHC)
  Tested dilution: -
  View 2 publications

• Flow Cytometry (Flow)
  Tested dilution: 0.5 µg/test
  View 71 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660
• APC, APC-eFluor 780
• Biotin
• Brilliant UV, Violet, and Yellow series
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. Its cytoplasmic domain possesses tyrosine phosphatase activity, essential for lymphocyte activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume)
• Optimization: Titrate for best assay performance
• Fluorochrome Information:
  • Alexa Fluor® 700 emits at 723 nm
  • Excitation: 633–647 nm
  • Laser: Red
  • Suggested optics: 685 LP mirror, 710/20 filter
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells and constitutes about 10% of lymphocyte surface proteins. It regulates Src-family kinases and plays a critical role in lymphocyte development, antigen signaling, and cytokine receptor regulation. CD45 isoforms arise via alternative splicing, differing in extracellular domains. CD45 is absent on non-hematopoietic cells and tissues, both normal and malignant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지