
Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Alexa Fluor 700, eBioscience
마우스 CD45 모든 아이소폼을 인식하는 30-F11 단클론 항체로, Alexa Fluor 700 형광 표지. Flow cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 가능. 적혈구 및 혈소판을 제외한 모든 조혈세포에 발현된 CD45 검출에 적합. 연구용으로만 사용.
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Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Alexa Fluor 700, eBioscience
Applications
Immunohistochemistry (IHC)
Tested dilution: -
View 2 publicationsFlow Cytometry (Flow)
Tested dilution: 0.5 µg/test
View 71 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Bacteria, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 700 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-F11 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_891456 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660
- APC, APC-eFluor 780
- Biotin
- Brilliant UV, Violet, and Yellow series
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue, Red, UV, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. Its cytoplasmic domain possesses tyrosine phosphatase activity, essential for lymphocyte activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume)
- Optimization: Titrate for best assay performance
- Fluorochrome Information:
- Alexa Fluor® 700 emits at 723 nm
- Excitation: 633–647 nm
- Laser: Red
- Suggested optics: 685 LP mirror, 710/20 filter
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells and constitutes about 10% of lymphocyte surface proteins. It regulates Src-family kinases and plays a critical role in lymphocyte development, antigen signaling, and cytokine receptor regulation. CD45 isoforms arise via alternative splicing, differing in extracellular domains. CD45 is absent on non-hematopoietic cells and tissues, both normal and malignant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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