Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), Alexa Fluor 700, eBioscience
CD47 단백질을 인식하는 B6H12 클론의 마우스 단클론 항체로, Alexa Fluor 700으로 표지되어 있습니다. Flow Cytometry에 최적화되어 있으며 인간 세포에 반응합니다. 세포 표면 CD47 분석 및 억제 실험에 활용 가능합니다. 냉장 보관(4°C), 동결 금지.
- 카탈로그번호
- 56-0479-42
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (1.0 µg)/test | View 7 publications |
| Inhibition Assays (IA) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B6H12 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation/Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762484 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 700
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody B6H12 reacts with CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP (signal regulator protein) and integrins (CD51/CD61 and CD41/CD61).
These interactions mediate bi-directional signaling, influencing neural synaptic activity and macrophage phagocytic regulation.
CD47 also acts as a receptor for thrombospondin, mediating T cell activation or apoptosis depending on ligand levels.
Stimulation of CD47 by monoclonal antibodies can induce CD4⁺CD25⁻ suppressive activity and upregulate Foxp3 expression.
Expression is found in most hematopoietic cells (T, B, monocytes, platelets, erythrocytes) and non-hematopoietic cells.
This antibody has been reported to have neutralizing activity.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (1.0 µg)/test.
A test corresponds to staining in 100 µL final volume. Cell number may vary (10⁵–10⁸ cells/test).
Fluorochrome details:
- Excitation: 633–647 nm
- Emission: 723 nm
- Recommended laser: Red laser
- Suggested filter setup: 685 LP mirror, 710/20 band pass filter
Target Information
CD47 (integrin-associated protein, IAP) is a transmembrane glycoprotein that interacts with integrins and regulates their functions.
Engagement with ligands or counter receptors modulates signaling pathways, including G protein activation and calcium regulation during cell adhesion.
It serves as a receptor for thrombospondin’s C-terminal domain, influencing membrane transport and signal transduction.
CD47 interacts with CD172a (SIRPα) on macrophages to inhibit phagocytosis of CD47⁺ cells.
It also regulates cell migration, B cell adhesion, T cell activation, and neuronal development, being abundant in synapse-rich brain and retinal regions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.