Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 8 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 5C3 monoclonal antibody reacts with human CD40, a 45–50 kDa type I transmembrane glycoprotein. CD40, a member of the TNFR family, is expressed on B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. It regulates B cell development and maturation by inducing Ig isotype switching and, with IL-4, protects B cells from apoptosis while promoting proliferation.

Interaction of CD40 with its ligand CD154 (gp39) on T cells is critical for T-B cell crosstalk and immune regulation. The 5C3 antibody can be used for B cell activation in in vitro functional assays.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells
• Recommended Usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Fluorochrome Characteristics:
Alexa Fluor 700 emits at 723 nm and is excited by the red laser (633–647 nm). Recommended detection setup: 685 LP mirror and 710/20 band pass filter.

Excitation: 633–647 nm
Emission: 723 nm
Laser: Red Laser

Target Information

CD40 is a single-chain glycoprotein and a member of the TNF/NGF receptor superfamily, homologous to CD30. It is expressed on all B cells except plasma cells and also found on some epithelial cells, carcinomas, and lymphoid dendritic cells.

CD40 is essential in mediating immune and inflammatory responses, including:

• T cell-dependent immunoglobulin class switching
• Memory B cell development
• Germinal center formation

The transcription factor AKNA regulates CD40 expression, influencing cell interactions. CD40 interacts with adaptor protein TNFR2 for signal transduction and is implicated in amyloid-beta-induced microglial activation, relevant to Alzheimer’s disease. Two alternatively spliced transcript variants exist.
CD40 dysfunction is associated with Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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