Thermo Fisher Scientific TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• PE
• PE-Cyanine7
• Super Bright 436
• Request custom conjugation

Product Specific Information

The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. Toll-like receptors (TLRs) are type I transmembrane proteins involved in innate immunity and inflammation. TLR4 associates with MD-2 and CD14 to recognize and signal in response to lipopolysaccharide (LPS). In mice, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages and is downregulated upon LPS stimulation. TLR4 is defective in C3H/HeJ and C57BL/10ScCr mice, which are hyporesponsive to LPS.

The MTS510 antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa) and preferentially binds to the TLR4/MD-2 complex. Binding differs from the UT41 antibody, which recognizes TLR4 with or without MD-2 association.

Applications Reported: Flow cytometric analysis and inhibition of LPS-induced cytokine production.
Applications Tested: Tested by flow cytometry using mouse peritoneal exudate cells (≤1 µg/test). Optimal titration is recommended.
Storage and Handling: Use in sterile conditions.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, which includes TLRs. TLRs recognize pathogen-associated molecular patterns (PAMPs) and mediate innate and adaptive immune responses. TLR4, the main receptor for LPS, requires MD-2 binding for full activation. The species origin of MD-2 determines LPS response specificity; for example, human MD-2 expressed in mouse cells alters TLR4 responsiveness to LPS analogs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.