Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (7E.17G9), Functional Grade, eBioscience

Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (7E.17G9), Functional Grade

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell-specific molecule and a third member of the CD28/CTLA-4 family. ICOS is a 47–57 kDa homodimer expressed on activated T cells, providing costimulatory activity for T cell activation and proliferation, and is essential for humoral immune responses. ICOS binds to its ligand B7h/B7RP-1 on activated APCs, including B cells, and may play a protective role in inflammatory autoimmune diseases. It is also involved in Th2 cell development.

Applications Reported: Suitable for flow cytometric analysis and inhibition of ligand binding in functional assays.
Applications Tested: Tested by flow cytometric analysis of unstimulated and Con A-activated (3-day) mouse splenocytes. Recommended ≤0.125 µg/test (100 µL final volume). Cell number may vary (10^5–10^8 cells/test). Antibody titration is advised for optimal performance.

Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

ICOS (CD278) encodes a protein belonging to the CD28 and CTLA-4 receptor family. It functions as a homodimer and plays a crucial role in cell-cell signaling, immune response modulation, and regulation of cell proliferation. ICOS has been associated with disorders such as Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.