
Thermo Fisher Scientific TAF5 Polyclonal Antibody
TAF5 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 액상 형태로 제공되며, 4°C에서 보관합니다. 인간 TAF5 단백질의 N-말단(1–50 aa)을 면역원으로 사용하였습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of Human TAF5 (TAF5 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 100 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
TFIID is a general transcription factor that initiates preinitiation complex assembly through direct interaction with the TATA promoter element.
It is a multisubunit complex consisting of a small TATA-binding polypeptide and other TATA-binding protein (TBP)-associated factors (TAFs).
TAF II p100 (also known as TAF5 or TAFII100) is the third largest subunit of human TFIID.
It contains six WD40 repeats at the C-terminus and has an N-terminus capable of forming a flexible dimer.
TAF II p100 plays an important role in forming the scaffold crucial for the assembly of the TFIID complex and may be involved in stabilizing TAF interactions.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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