Thermo Fisher Scientific TAF5 Polyclonal Antibody
TAF5 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody입니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 인간 반응성으로 4°C 보관, 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of Human TAF5 (TAF5 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 100kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, predicted to react with Mouse
Target Information
TFIID is a general transcription factor initiating preinitiation complex assembly through interaction with the TATA promoter element. It consists of a small TATA-binding polypeptide and other TBP-associated factors (TAFs).
TAF II p100 (TAF5 or TAFII100) is the third largest subunit of human TFIID, containing six WD40 repeats at the C-terminus and an N-terminus forming a flexible dimer. It plays a key role in forming the scaffold for TFIID complex assembly and stabilizing TAF interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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