Thermo Fisher Scientific Cortactin Polyclonal Antibody
Thermo Fisher Scientific의 Cortactin Polyclonal Antibody는 Rat, Human, Mouse 등 다양한 종에 반응하며 Western blot과 IHC(P) 분석에 적합합니다. 액상 형태로 제공되며 항원 친화 크로마토그래피로 정제되었습니다. 세포 이동과 액틴 네트워크 연구에 활용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat, Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the carboxy-terminal residues of human cortactin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10986147 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Cortactin (Cttn) is a ubiquitous actin-binding protein originally identified as a substrate for Src. It contributes to actin cytoskeleton organization and cell shape, and plays an important role in lamellipodia formation and cell migration. Cortactin accumulates in actin-enriched peripheral structures, facilitating actin network formation. It contains four major domains: the N-terminal acidic (NTA), tandem repeats, proline-rich, and SH3 domains. NTA associates with the Arp2/3 and WASP complex at F-actin branches. Cortactin promotes cell motility and invasion, notably in invadopodia involved in ECM degradation by cancer cells. It is phosphorylated by Src family kinases at Y421, Y466, and Y482, and by Erk family kinases at S405 and S418.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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