Thermo Fisher Scientific GFP Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.

As a fusion tag, GFP allows localization studies of proteins, tracking their movement, and investigating subcellular compartment dynamics. GFP technology has provided significant insights into the physiological activities of living cells.

GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without requiring additional proteins or cofactors. GFP fluorescence is stable, species-independent, and suitable for a variety of applications, including:

• Monitoring gene expression and protein localization
• Assessing protein-protein interactions (e.g., yeast two-hybrid system)
• Measuring distances between proteins in FRET experiments

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.