Thermo Fisher Scientific PIK3C2A Monoclonal Antibody (OTI3F8), TrueMAB
PIK3C2A 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western Blot 및 IHC(Paraffin) 실험에 적합. Human 시료 반응성. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 함유. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 230–560 of human PIK3C2A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The Class II PI3 kinases, including PIK3C2A, phosphorylate the 3 position of PI or PI4P. These enzymes are associated with receptor-mediated processes such as insulin signaling, neuronal survival, and growth factor signaling.
PI3-kinases are involved in pathways related to cell proliferation, oncogenic transformation, survival, migration, and intracellular trafficking.
PIK3C2A contains a lipid kinase catalytic domain and a C-terminal C2 domain, which mediates calcium-dependent phospholipid binding and protein-protein interactions.
Its kinase activity is not sensitive to nanomolar levels of wortmannin and can be activated by insulin, suggesting involvement in integrin-dependent signaling.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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