Thermo Fisher Scientific BMI-1 Monoclonal Antibody (3E3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

in situ PLA (PLA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3E3

Immunogen

Purified recombinant fragment of human BMI1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11152159

Product Specific Information

MA5-15919 targets BMI1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15919 immunogen is purified recombinant fragment of human BMI1 expressed in E. Coli. .

MA5-15919 detects BMI1 which has a predicted molecular weight of approximately 37kDa.

Target Information

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A Lys-119, rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.