Thermo Fisher Scientific ZBED1 Monoclonal Antibody (OTI5A2), TrueMAB
인간 ZBED1 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, FACS 분석에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 세포 증식 및 DNA 복제 관련 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5A2 |
| Immunogen | Full-length human recombinant ZBED1 protein produced in HEK293T cells. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ZBED1 (DREF) is the human homolog of the Drosophila DNA replication-related element-binding factor (DREF).
It functions as a transcriptional regulator required for expression of genes involved in DNA replication and cell proliferation.
RNA interference (RNAi) targeting ZBED1 inhibits S-phase entry and reduces histone H1 mRNA in HeLa cells, indicating its role in human cell proliferation.
At least 22 ribosomal protein genes contain ZBED1 binding sites in their promoters, and ZBED1 knockdown decreases RP gene expression.
Immunoprecipitation studies show that ZBED1 self-associates via a carboxyl-terminal hATC domain, which is essential for nuclear localization and DNA binding.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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