Thermo Fisher Scientific BRAF (V600E Mutant Specific) (Prognostic Marker) Monoclonal Antibody (V600E/1321)
BRAF(V600E) 변이 특이적 단클론 항체로, 인간 시료에 반응하며 ELISA 등 다양한 연구용 분석에 사용 가능. 단백질 A/G로 정제된 액상 항체로, 4°C 보관. MAPK/ERK 신호전달 관련 암 연구에 적합.
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Applications
- ELISA (ELISA): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | V600E/1321 |
| Immunogen | An eleven amino acid residue long synthetic peptide (aa596-606), corresponding to Cys-GLAT(E)KSRWSG from human BRAF protein. It was conjugated to KLH at the N-terminus. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
The B-RAF (BRAF) gene encodes a serine/threonine protein kinase belonging to the RAF family. This protein regulates the MAP kinase/ERK signaling pathway, influencing cell division, differentiation, and secretion.
Mutations, particularly V600E, are frequently observed in melanoma and various other cancers, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia, and lung adenocarcinoma.
Mutations are also linked to cardiofaciocutaneous, Noonan, and Costello syndromes, which share overlapping phenotypes. A pseudogene of BRAF is located on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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