Thermo Fisher Scientific Human RIP1 Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 14 amino acids near the carboxy terminus of human RIP1.
PEP-1599 can be used as a blocking peptide with polyclonal antibody PA5-34556.
For use as a blocking peptide in Western blot, incubate the peptide with equal volume of antibody for 30 min at 37ºC.

Target Information

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage.
Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor.
Phosphorylates DAB2IP at Ser-728 in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade.
Ubiquitination by TRAF2 via Lys-63-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules.
Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation.
Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death.
RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.