Thermo Fisher Scientific NCoR2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

4 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:200

-

Immunoprecipitation (IP)

-

View 2 publications 2 publications

ChIP assay (ChIP)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NCoR2,uniProtId:Q9Y618-1,ncbiNodeId:9606,antigenRange:95-565,antigenLength:2514,antigenImageFileName:PA1-843_NCoR2_Q9Y618-1_Rabbit.svg,antigenImageFileNamePDP:PA1-843_NCoR2_Q9Y618-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2149135

Product Specific Information

PA1-843 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human and mouse cells.

PA1-843 has been successfully used in Western blot, ICC/IF and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects an ~250 kDa protein representing SMRT from HeLa cell nuclear extracts.

The PA1-843 antigen is His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.

Target Information

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.