Thermo Fisher Scientific Soluble Guanylyl Cyclase beta 1 Polyclonal Antibody
Soluble Guanylyl Cyclase β1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, IHC, ICC/IF, ELISA, IP, IM 등 다양한 응용에 사용 가능. 고순도 친화 크로마토그래피 정제 제품으로, -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 175–225 on rat guanylate cyclase soluble subunit beta-1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | –20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase: a soluble form (GCS or sGC), which acts as a receptor for nitric oxide, and a membrane-bound receptor form (GC), which serves as a peptide hormone receptor.
The GC-C protein consists of an extracellular domain, a single transmembrane domain, and a cytoplasmic region with kinase-like and catalytic domains. It is expressed as two glycosylated forms: a 130 kDa precursor in the endoplasmic reticulum and a 145 kDa mature form on the plasma membrane.
Ligand binding to the extracellular domain of GC-C promotes cGMP accumulation. GC-C functions as the receptor for heat-stable enterotoxins secreted by pathogenic E. coli strains causing secretory diarrhea, and also binds guanylin and uroguanylin peptides that regulate renal function in response to oral salt load.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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