Thermo Fisher Scientific Soluble Guanylyl Cyclase beta 1 Polyclonal Antibody, FITC
FITC로 표지된 Soluble Guanylyl Cyclase beta 1 폴리클로날 항체로, Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. Rabbit IgG로 제작되었으며, Human, Mouse, Rat 등 다양한 종에 반응. Affinity chromatography로 정제된 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 175–225 on rat guanylate cyclase soluble subunit beta-1 protein |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase: a soluble form (sGC) acting as a receptor for nitric oxide, and a membrane-bound receptor form (GC) that functions as a peptide hormone receptor.
The GC-C protein consists of an extracellular domain, a single transmembrane domain, and a cytoplasmic region with kinase-like and catalytic domains. It exists as two glycosylated forms: a 130 kDa precursor in the endoplasmic reticulum and a 145 kDa mature form on the plasma membrane.
Ligand binding to GC-C promotes cGMP accumulation. GC-C serves as a receptor for heat-stable enterotoxins from certain pathogenic E. coli strains and also binds guanylin and uroguanylin peptides involved in renal function regulation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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