Thermo Fisher Scientific CD73 (Immuno-Oncology Target) Monoclonal Antibody (NT5E/2503)
인간 CD73 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, 면역종양학 연구에 적합합니다. WB, IHC, ICC, Flow Cytometry 등 다양한 응용 가능. 단백질 A/G로 정제된 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NT5E/2503 |
| Immunogen | Recombinant full-length human NT5E protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD73 (Ecto-5'-nucleotidase, 5'-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5'-mononucleotides to nucleosides at neutral pH, with AMP as the preferred substrate. It forms a dimer of two identical 70 kDa subunits attached externally to the plasma membrane via a glycosyl phosphatidylinositol linkage.
CD73 serves as a marker of lymphocyte differentiation, and its deficiency is linked to various immunodeficiency diseases. Other 5'-nucleotidase forms exist in the cytoplasm and lysosomes, differing by substrate affinities and activation requirements. The CD73 gene is localized to chromosome 6q14-q21. Defects may cause joint and artery calcification and intestinal tuberculosis. Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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