Thermo Fisher Scientific Phospho-STAT3 (Tyr705) Monoclonal Antibody (LUVNKLA), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
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Product Specifications

Available Formats:

• APC
• eFluor 450
• FITC
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This LUVNKLA monoclonal antibody recognizes human and mouse STAT3 phosphorylated at tyrosine 705 (Y705). STAT3 is part of the STAT family of transcription factors involved in apoptosis, differentiation, and proliferation. Activation occurs via cytokine receptor–associated JAK kinases, leading to nuclear translocation and gene regulation.

STAT3 is activated downstream of cytokines such as interferons, IL-5, IL-6, IL-10, and LIF, and is crucial for Th17 cell differentiation, growth, and survival. Dysregulation of STAT3 activity is linked to cancer progression and immune dysfunction.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated for use at 5 µL (0.125 µg)/test. One test = amount required to stain cells in 100 µL volume (10⁵–10⁸ cells/test).

Recommended Protocol:
Use Protocol C (Two-step Fixation/Methanol).
Protocols A and B are not compatible.
See Staining Intracellular Antigens for Flow Cytometry Protocol in BestProtocols® for details.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (#00-8222) or 1-step Fix/Lyse Solution (#00-5333) for up to 3 days at 4°C in the dark.
Excitation/Emission: 488–561 nm / 775 nm (Blue, Green, Yellow-Green Laser).
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

STAT3 (Signal Transducer and Activator of Transcription 3) is a transcription factor activated by phosphorylation at Tyr705 by JAK1/2. It mediates gene expression in response to cytokines and growth factors (e.g., IFN-α, IFN-γ, EGF, PDGF, IL-6). STAT3 regulates cell growth and apoptosis and is modulated by coactivators such as CREB-binding protein.
The STAT3 gene is located on chromosome 17q. Knockout of STAT3 is embryonically lethal, emphasizing its essential role in cellular signaling.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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