Thermo Fisher Scientific Caspase-9 Monoclonal Antibody (1D7)

Applications and Tested Dilution

Product Specifications

Target Information

Caspase-9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 containing a CARD domain. Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis by proteolysis of specific substrates.

Caspase-9 is active as a heterotetramer, present in the cytosol, and translocates to mitochondria upon activation. It participates in the caspase activation cascade responsible for apoptosis execution by cleaving and activating Caspase-3 and Caspase-6.

Binding of Caspase-9 to Apaf-1 leads to activation of the protease, which then cleaves and activates Caspase-3. Caspase-9 promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and cleaves poly(ADP-ribose) polymerase (PARP).

Caspase-9 is expressed in various human tissues. In rats, an isoform with an alternative C-terminal peptide sequence has been identified. Mutations in Caspase-9 affect brain and neuron development. In humans, tissue-specific dysfunction of Caspase-9 expression is observed, and low levels may contribute to cancer and neurodegenerative diseases such as Alzheimer’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.