Thermo Fisher Scientific MEKK3 Monoclonal Antibody (5C1)
MEKK3 단백질을 표적하는 Rabbit 모노클로널 항체로, WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합합니다. Unconjugated 형태이며 1 mg/mL 농도로 제공됩니다. 연구용으로 MAPK 및 ERK 경로 분석에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:300 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C1 |
| Immunogen | Human MEKK3 between 300 to 600 amino acids |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Target Information
MAP3K3 (MEKK3) directly regulates MAPK8 and extracellular signal-regulated protein kinase (ERK) pathways by phosphorylating multiple MAP2Ks. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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