Thermo Fisher Scientific ARL3 Monoclonal Antibody (OTI3D3), TrueMAB
인간 ARL3 단백질(aa 2-182)을 인식하는 Mouse IgG2a 단일클론 항체로 Western blot에 적합. TrueMAB 형식의 고순도 항체로 PBS/BSA/glycerol buffer에 보존. 연구용으로만 사용 가능하며 -20°C 보관.
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Thermo Fisher Scientific ARL3 Monoclonal Antibody (OTI3D3), TrueMAB
Applications
- Western Blot (WB)
Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3D3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2–182 of human ARL3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily.
The predicted 182-amino acid ARL3 (ADP-ribosylation-like factor) protein shares 97% amino acid identity with rat ARLl3 and 43% identity with human ARF1.
Like ARFs, ARL3 has a glycine at position 2 (site of N-myristoylation) and lacks cysteine residues near the C terminus found in other RAS family members.
Northern blot analysis detected a 1-kb ARL3 transcript in all tissues tested, with highest expression in heart and lung, and lower expression in brain, liver, kidney, ovary, and testis.
A 5.5-kb transcript was also detected in most tissues, with highest expression in brain.
Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells.
Although ARL3 binds GTP, it is devoid of activity in cholera toxin-dependent ADP-ribosylation of Gs, and is therefore classified as an ARF-like protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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