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카탈로그 번호 | 설명 | 상태 | 단위 | 가격 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
3735A | Takara 3735A CellAmp™ Direct TB Green® RT-qPCR Kit, Each pk | 재고문의 | pk | 1,186,000원 | 1,304,600원 | |
3737A | Takara 3737A CellAmp™ Direct Lysis and RT set, Each pk | 재고문의 | pk | 759,000원 | 834,900원 | |
3735S | Takara 3735S CellAmp™ Direct TB Green® RT-qPCR Kit, Each pk | 재고문의 | pk | 315,000원 | 346,500원 | |
3737S | Takara 3737S CellAmp™ Direct Lysis and RT set, Each pk | 재고문의 | pk | 189,000원 | 207,900원 |
Overview
- High-throughput analyses can be performed easily and rapidly (~1.5 hr)—there is no need to do RNA extraction or a heat-treatment to inactivate DNase, making the process faster and less cumbersome
- Can be used with cells differentiated from stem cells and iPS cells—the improved lysis buffer works well with a variety of adherent cells, floating cells, primary cultured cells, various stem cells, iPS cells, etc.
- Lysates can be stored for a long time—storage stability has been confirmed at –20°C for at least 6 months
- Resistance to PCR inhibitors and high specificity—use of TB Green Fast qPCR Mix as the real-time PCR reagent makes this product highly resistant to PCR inhibitors and highly reactive for high GC-content targets
More Information
Applications
- High-throughput gene expression analyses in a very short time (~1.5 hr)
- RT-qPCR of stem cells (such as iPS cells, floating cells, adherent cells, primary cells, etc.)
- Analysis of samples with high GC content-high tolerance to inhibitors and high specificity
- Analysis of samples typically impacted by genomic DNA contamination
Components
3735A (100 reactions) | 3735S (20 reactions) | |
CellAmp Washing Buffer | 2.5 ml x 5 | 2.5 ml |
CellAmp Lysis Buffer II | 1 ml x 5 | 1 ml |
DNase I for Direct RNA Prep | 200 μl | 40 μl |
Stop Solution | 250 μl | 50 μl |
PrimeScript RT Enzyme Mix | 200 μl | 40 μl |
5X CellAmp Buffer II | 400 μl | 80 μl |
RT Primer Mix | 100 μl | 20 μl |
RNase Free H2O | 1 ml x 2 | 1 ml |
TB Green Fast qPCR Mix (2X) (3735A/S) | 625 μl x 10 | 625 μ x 2 |
ROX Reference Dye (50X) | 250 μl | 50 μl |
ROX Reference Dye II (50X) | 250 μl | 50 μl |
Storage
–20°C
Additional Product Information
Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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