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Takara CellAmp Direct TB Green RT-qPCR Kit
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Takara CellAmp Direct TB Green RT-qPCR Kit

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RNA 추출 없이 빠른 RT-qPCR 수행 가능. 다양한 세포 유형(줄기세포, iPS 세포 등)에 적용. DNase 처리 불필요로 작업 단순화. -20°C에서 6개월 이상 안정 저장. PCR 억제물 저항성과 높은 특이성 제공.

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RT PCR Kits
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pk
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Takara 3735A CellAmp™ Direct TB Green® RT-qPCR Kit, Each pk판매 단위 pk ·
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Takara 3735S CellAmp™ Direct TB Green® RT-qPCR Kit, Each pk판매 단위 pk ·
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Takara 3737A CellAmp™ Direct Lysis and RT set, Each pk판매 단위 pk ·
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Takara 3737S CellAmp™ Direct Lysis and RT set, Each pk판매 단위 pk ·
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Takara · Takara CellAmp Direct TB Green RT-qPCR Kit

Overview

  • High-throughput analyses in ~1.5 hr — No RNA extraction or DNase inactivation required, enabling rapid and simplified workflows.
  • Compatible with various cell types — Improved lysis buffer supports adherent cells, floating cells, primary cultured cells, stem cells, and iPS cells.
  • Stable lysates — Storage confirmed at –20°C for at least 6 months.
  • High resistance and specificity — Utilizes TB Green Fast qPCR Mix for strong resistance to PCR inhibitors and high reactivity with GC-rich targets.

Applications

  • High-throughput gene expression analysis (~1.5 hr)
  • RT-qPCR of stem cells (iPS, floating, adherent, primary cells, etc.)
  • Analysis of high GC-content samples with high inhibitor tolerance and specificity
  • Samples affected by genomic DNA contamination

Components

Component 3735A (100 reactions) 3735S (20 reactions)
CellAmp Washing Buffer 2.5 ml × 5 2.5 ml
CellAmp Lysis Buffer II 1 ml × 5 1 ml
DNase I for Direct RNA Prep 200 μl 40 μl
Stop Solution 250 μl 50 μl
PrimeScript RT Enzyme Mix 200 μl 40 μl
5X CellAmp Buffer II 400 μl 80 μl
RT Primer Mix 100 μl 20 μl
RNase Free H₂O 1 ml × 2 1 ml
TB Green Fast qPCR Mix (2X) 625 μl × 10 625 μl × 2
ROX Reference Dye (50X) 250 μl 50 μl
ROX Reference Dye II (50X) 250 μl 50 μl

Storage

  • Store at –20°C

Additional Product Information

Refer to the product’s Certificate of Analysis for details on storage conditions, components, and technical specifications.
The Kit Components List is available under the Documents tab.

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