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Takara CellAmp Direct TB Green RT-qPCR Kit
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RNA 추출 없이 빠른 RT-qPCR 수행 가능. 다양한 세포 유형(줄기세포, iPS 세포 등)에 적용. DNase 처리 불필요로 작업 단순화. -20°C에서 6개월 이상 안정 저장. PCR 억제물 저항성과 높은 특이성 제공.
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카탈로그 번호 · 상품 설명출고판매가담기
Takara 3735A CellAmp™ Direct TB Green® RT-qPCR Kit, Each pk판매 단위 pk ·
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Takara 3735S CellAmp™ Direct TB Green® RT-qPCR Kit, Each pk판매 단위 pk ·
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Takara 3737A CellAmp™ Direct Lysis and RT set, Each pk판매 단위 pk ·
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Takara 3737S CellAmp™ Direct Lysis and RT set, Each pk판매 단위 pk ·
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Takara · Takara CellAmp Direct TB Green RT-qPCR Kit
Overview
- High-throughput analyses in ~1.5 hr — No RNA extraction or DNase inactivation required, enabling rapid and simplified workflows.
- Compatible with various cell types — Improved lysis buffer supports adherent cells, floating cells, primary cultured cells, stem cells, and iPS cells.
- Stable lysates — Storage confirmed at –20°C for at least 6 months.
- High resistance and specificity — Utilizes TB Green Fast qPCR Mix for strong resistance to PCR inhibitors and high reactivity with GC-rich targets.
Applications
- High-throughput gene expression analysis (~1.5 hr)
- RT-qPCR of stem cells (iPS, floating, adherent, primary cells, etc.)
- Analysis of high GC-content samples with high inhibitor tolerance and specificity
- Samples affected by genomic DNA contamination
Components
| Component | 3735A (100 reactions) | 3735S (20 reactions) |
|---|---|---|
| CellAmp Washing Buffer | 2.5 ml × 5 | 2.5 ml |
| CellAmp Lysis Buffer II | 1 ml × 5 | 1 ml |
| DNase I for Direct RNA Prep | 200 μl | 40 μl |
| Stop Solution | 250 μl | 50 μl |
| PrimeScript RT Enzyme Mix | 200 μl | 40 μl |
| 5X CellAmp Buffer II | 400 μl | 80 μl |
| RT Primer Mix | 100 μl | 20 μl |
| RNase Free H₂O | 1 ml × 2 | 1 ml |
| TB Green Fast qPCR Mix (2X) | 625 μl × 10 | 625 μl × 2 |
| ROX Reference Dye (50X) | 250 μl | 50 μl |
| ROX Reference Dye II (50X) | 250 μl | 50 μl |
Storage
- Store at –20°C
Additional Product Information
Refer to the product’s Certificate of Analysis for details on storage conditions, components, and technical specifications.
The Kit Components List is available under the Documents tab.
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