Thermo Fisher Scientific Phospho-AKT1/AKT3 (Tyr437, Tyr434) Polyclonal Antibody
Human, Mouse, Rat에 반응하는 Rabbit Polyclonal Antibody로 AKT1/AKT3 Tyr437/Tyr434 인산화 부위를 인식합니다. Western blot 및 IHC(P) 검증 완료. 항원 친화 크로마토그래피로 정제되었으며 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific Phospho-AKT1/AKT3 (Tyr437, Tyr434) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 437 (T-R-Y(p)-F-D) derived from Human AKT1/3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2608972 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
AKT1 is one of three closely related serine/threonine-protein kinases (AKT1, AKT2, and AKT3) collectively known as AKT kinase. These kinases regulate various cellular processes including metabolism, proliferation, cell survival, growth, and angiogenesis through phosphorylation of downstream substrates. AKT mediates insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface, facilitating glucose uptake. Phosphorylation of PTPN1 at Ser-50 negatively modulates its phosphatase activity, preventing dephosphorylation of the insulin receptor and attenuation of insulin signaling.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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