Thermo Fisher Scientific Phospho-AKT1/AKT3 (Tyr437, Tyr434) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of tyrosine 437 (T-R-Y(p)-F-D) derived from Human AKT1/3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKT1/AKT3,uniProtId:P31749-1,ncbiNodeId:9606,antigenRange:435-439,antigenLength:480,antigenImageFileName:PA5-40162_AKT1AKT3_P31749-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40162_AKT1AKT3_P31749-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AKT1/AKT3,uniProtId:Q9Y243-1,ncbiNodeId:9606,antigenRange:435-439,antigenLength:479,antigenImageFileName:PA5-40162_AKT1AKT3_Q9Y243-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40162_AKT1AKT3_Q9Y243-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2608972

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at Ser-50 negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.