Thermo Fisher Scientific Phospho-RIP2 (Ser176) Polyclonal Antibody
Phospho-RIP2 (Ser176) 폴리클로날 항체는 인간 및 생쥐 RIPK2 단백질의 Ser176 인산화 부위를 인식합니다. Western blot 및 IHC(P) 실험에 적합하며, 항원 친화 크로마토그래피로 정제된 고순도 항체입니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Phospho-RIP2 (Ser176) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 176 (S-L-S(p)-Q-S) derived from Human RIPK2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2610276 |
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
RIPK2 is a serine/threonine kinase involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize, and recruit RIPK2 through CARD-CARD domains. RIPK2 contributes to tyrosine phosphorylation of ARHGEF2 via Src kinase, leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes Lys-63-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2, and BIRC3. The polyubiquitinated RIPK2 mediates recruitment of MAP3K7/TAK1 to IKBKG/NEMO, induces Lys-63-linked polyubiquitination of IKBKG/NEMO, and activates IKBKB/IKKB. This cascade releases NF-kappaB from inhibitors, enabling nuclear translocation and transcriptional activation of genes involved in immune response, growth control, and apoptosis protection. RIPK2 also promotes BCL10 phosphorylation and NF-kappaB activation during T-cell receptor engagement.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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