Thermo Fisher Scientific KPNB1 Polyclonal Antibody
KPNB1 단백질을 인식하는 염소 유래 폴리클로날 항체로, Western blot, IHC, ICC, Flow Cytometry에 사용 가능. 인간 및 랫트 반응성. 합성 펩타이드 면역원 사용. 0.5 mg/mL 농도의 액상 형태로 제공되며, -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.01–0.1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (RRSKTNKAKTLAT) corresponding to the C-terminus amino acids of KPNB1 (aa 851–864) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10980020 |
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, and rat based on sequence homology.
Tested in Peptide ELISA with an antibody detection limit dilution of 1:32,000.
Target Information
Nucleocytoplasmic transport is a signal- and energy-dependent process occurring through nuclear pore complexes in the nuclear envelope. Proteins containing a nuclear localization signal (NLS) are imported via a receptor complex composed of importin alpha and beta (karyopherins). Importin alpha binds NLS-containing cargo, while importin beta docks the complex to the nuclear pore. In the presence of nucleoside triphosphates and Ran GTPase, the complex translocates through the pore, after which importin subunits dissociate. Importin beta remains at the pore, while importin alpha and the cargo enter the nucleoplasm. Interactions between importin beta and FG repeats of nucleoporins are essential for translocation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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