Thermo Fisher Scientific NSE Monoclonal Antibody (04)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1:200–1:500

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human ENO2.

Target Information

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis.
NSE is stable in biological fluids and diffuses to extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.

There are three mammalian enolases (ENO1, ENO2, ENO3), also known as enolase alpha, beta, and gamma:

• Alpha-subunit (ENO1): Expressed in most tissues
• Beta-subunit (ENO3): Expressed only in muscle
• Gamma-subunit (ENO2): Expressed primarily in neurons and neuroendocrine cells

Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms.
Neurons, requiring high energy, are rich in glycolytic enzymes such as GAPDH and NSE.
Antibodies to NSE protein are useful for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells.

Release of NSE from damaged neurons into CSF and blood serves as a biomarker of neuronal injury.
Clinically, NSE is used as a sensitive marker of neuronal damage in disorders such as:

• Stroke
• Hypoxic brain damage
• Status epilepticus
• Creutzfeldt-Jakob disease
• Herpetic encephalitis

Elevated NSE concentrations are also observed in plasma and neoplasias such as pediatric neuroblastoma and small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.