Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546
Alexa Fluor 546으로 결합된 염소 유래 항-토끼 IgG(F(ab`)2) 2차 항체로, WB 및 ICC/IF에 적합합니다. 높은 특이성과 신호 증폭 효과를 제공하며, 다양한 형광 검출 시스템과 호환됩니다. 4°C 암소 보관, 연구용 전용입니다.
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Applications and Tested Dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 546 |
| Excitation / Emission Max | 561 / 572 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2534115 |
| Target | IgG |
| Cross Adsorption | Against pooled human serum, mouse serum, mouse plasmacytoma/hybridoma proteins, and purified human paraproteins |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
- Product will be shipped at room temperature.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies offer increased versatility, enabling users to use many detection systems (e.g., HRP, AP, fluorescence).
They can also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
- Alexa Fluor 350, 405, 430, 488, 514, 532, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790
- Alexa Fluor Plus 488, 555, 594, 647
- AP, APC, Biotin, Biotin-XX, Cyanine3, Cyanine5
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 755, 800
- FITC, HRP, Pacific Blue, Pacific Orange, PE, PE-Alexa Fluor 610, PE-Alexa Fluor 647, Rhodamine, Rhodamine Red-X, Texas Red, Texas Red-X, TRITC
- Request custom conjugation available
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