Thermo Fisher Scientific Neomycin Phosphotransferase II Monoclonal Antibody (4B4D1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

4B4D1

Immunogen

Purified recombinant fragment of NPT expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10979669

Product Specific Information

MA5-15275 targets NPT in indirect ELISA, WB applications and shows reactivity with Human samples.

The MA5-15275 immunogen is purified recombinant fragment of NPT expressed in E. Coli.

Target Information

Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3-phosphotransferase (denoted aph(3)-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.