
Norgen Bacillus cereus TaqMan PCR Kit Dx
Bacillus cereus 검출용 TaqMan PCR 키트로 CE-IVDD 인증을 받은 진단용 제품입니다. 24회 반응용으로 마스터믹스, 프라이머·프로브, 양·음성 대조군 포함. FAM 및 HEX 채널 기반의 PCR 결과 확인 가능하며, -20°C 보관이 필요합니다.
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Norgen Bacillus cereus TaqMan PCR Kit Dx
SKU: DxTM36900
Features and Benefits
- Detection kits for Bacillus cereus
- CE-IVDD marked in accordance with the European Commission Directive 98/79/EC for in vitro diagnostic use
- Research version available
- Available in TaqMan format for analysis
Product Description
Bacillus cereus is a rod-shaped, gram-positive bacterium. Some strains of B. cereus cause food poisoning and other diseases such as keratitis. B. cereus grows at a wide range of temperatures, from 4°C to 37°C. Many foodborne illnesses caused by B. cereus result from improperly cooked or stored food, including dairy products and meats. The majority of B. cereus food poisoning can be divided into two symptom types – emetic and diarrheal – both caused by enterotoxins produced by the bacteria.
Kit Configuration
Bacillus cereus TaqMan PCR Kit, 24 reactions
- Ready-to-use format including Master Mix for target and PCR control
- Specific Primer and Probe mix for pathogen/virus/viroid of interest
- Positive and negative controls included to confirm reagent integrity
Supporting Data
Figure 1. TaqMan PCR Positive Result
Both PCR signals above the baseline from FAM and HEX channels indicate a successful PCR amplification.
OCR 추출 내용:
- 그래프 제목: Amplification
- 상단 그래프: FAM channel (Y축: RFU, X축: Cycles)
- 하단 그래프: HEX channel (Y축: RFU, X축: Cycles)
Figure 2. TaqMan PCR Negative Result
No target DNA detected in the FAM channel, but amplification signal from HEX indicates a successful PCR.
OCR 추출 내용:
- 상단: Amplification (FAM channel, RFU vs Cycles)
- 하단: Amplification (HEX channel, RFU vs Cycles)
- X축: Cycles / Y축: RFU
Figure 3. TaqMan PCR Inhibition Result
No signal detected in both FAM and HEX channels. Re-sample preparation is suggested using the recommended DNA purification kit.
OCR 추출 내용:
| 항목 | 내용 |
|------|------|
| 그래프 제목 | Amplification |
| 채널 | FAM channel / HEX channel |
| X축 | Cycles (0~40) |
| Y축 | RFU (-100~900) |
Storage Conditions
The Bacillus cereus TaqMan PCR Kit Dx is shipped on dry ice.
All components should be stored at -20°C upon arrival.
Avoid repeated freeze-thaw cycles (>3x) of the Master Mix and Positive Control.
If used intermittently, aliquot and freeze reagents accordingly.
Kit Components
| Component | TaqMan Probe Cat. DxTM36900 (24 rxns) |
|---|---|
| MDx TaqMan 2X PCR Master Mix Dx | 550 µL |
| Bacillus cereus Primer & Probe Mix | 2 × 70 µL |
| Bacillus cereus Positive Control Dx (200,000 copies/μL) | 50 µL |
| Nuclease-Free Water | 1.25 mL |
| Product Insert | 1 |
Documentation
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