Thermo Fisher Scientific ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4H6L2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ChIP assay (ChIP)

-

View 6 publications 6 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Pig, Plant

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

4H6L2

Immunogen

Protein corresponding to human ATP Citrate Lyase [aa1-aa425] if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATP Citrate Lyase,uniProtId:P53396-1,ncbiNodeId:9606,antigenRange:1-425,antigenLength:1101,antigenImageFileName:702731_ATP_Citrate_Lyase_P53396-1_Rabbit.svg,antigenImageFileNamePDP:702731_ATP_Citrate_Lyase_P53396-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2716887

Product Specific Information

This antibody is predicted to react with Monkey, Sheep, Cat

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.