Thermo Fisher Scientific ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4H6L2)
ATP Citrate Lyase 단백질을 인식하는 재조합 토끼 단클론 항체. Western blot, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며, lot 간 일관성이 우수. 연구용으로 인간 및 다양한 종에 반응.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | – |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | – |
| Flow Cytometry (Flow) | – | 2 publications |
| ChIP assay (ChIP) | – | 6 publications |
| Functional Assay | – | 1 publication |
| In vitro Assay | – | 1 publication |
| Miscellaneous PubMed | – | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Pig, Plant |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4H6L2 |
| Immunogen | Protein corresponding to human ATP Citrate Lyase [aa1–aa425] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2716887 |
Product Specific Information
This antibody is predicted to react with Monkey, Sheep, and Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. It is a tetramer (approx. 440 kDa) composed of identical subunits. The enzyme catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with the hydrolysis of ATP to ADP and phosphate. Acetyl-CoA produced serves key biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate lyase may contribute to acetylcholine biosynthesis. Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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