Thermo Fisher Scientific PCNA Polyclonal Antibody
PCNA 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 polyclonal 항체. Western blot과 IHC(P)에서 검증됨. DNA 복제 및 수선 과정 연구에 적합하며, 세포 증식 마커로 활용 가능. -20°C 보관, 동결/해동 반복 피할 것.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.03–0.1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence C-EAPNQEKVSDYE |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2933570 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
PCNA (Proliferating Cell Nuclear Antigen) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways.
It binds to CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and is useful for assessing tumor proliferation status.
It is localized in the nucleus, functions as a cofactor of DNA polymerase delta, and forms a homotrimer to enhance DNA synthesis during replication.
Upon DNA damage, PCNA is ubiquitinated and participates in the RAD6-dependent DNA repair pathway.
Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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