Thermo Fisher Scientific HEF1 Polyclonal Antibody
HEF1 단백질을 표적으로 하는 Thermo Fisher Scientific의 goat polyclonal antibody. 인간 반응성, Western blot 검증 완료. 세포 이동, 성장, 아폽토시스 관련 연구에 적합. 액상 형태, -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–0.3 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (NKPQNKCDDLDR) corresponding to internal amino acids of NEDD9 (aa 519–531) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10987514 |
Product Specific Information
- Predicted to react with canine, mouse, and rat based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 8,000.
Target Information
HEF1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis, and oncogenic transformation.
The Cas family of docking proteins are substrates of focal adhesion kinase (FAK) and Src family tyrosine kinases, both active drug development targets.
HEF1 overexpression increases mRNA levels encoding motility and transformation-related proteins, including metalloproteinases, MLCK, p160ROCK, and ErbBi.
HEF1 also mediates apoptosis in epithelial-derived cell lines (e.g., MCF7, HeLa).
Overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance, highlighting the significance of this family in cancer prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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