Thermo Fisher Scientific COX4I2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (RWDYEKKQWKK) corresponding to the C-terminus amino acids of COX4I2 (aa 161-171). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:COX4I2,uniProtId:Q96KJ9-1,ncbiNodeId:9606,antigenRange:161-171,antigenLength:171,antigenImageFileName:PA5-18760_COX4I2_Q96KJ9-1_Goat.svg,antigenImageFileNamePDP:PA5-18760_COX4I2_Q96KJ9-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10983473

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 2,000.

Target Information

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunbit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [Uniprot]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.