Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 661
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The M5/114.15.2 monoclonal antibody reacts with mouse MHC class II molecules (I-A and I-E subregion-encoded glycoproteins: I-Aᵇ, I-Aᵈ, I-Aᵠ, I-Eᵈ, I-Eᵏ). It detects polymorphic determinants on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying H-2ᵇ, H-2ᵈ, H-2ᵠ, H-2ᵖ, H-2ʳ, and H-2ᵘ haplotypes, but not from H-2ˢ or H-2ᶠ.

This antibody is suitable for flow cytometric analysis of mouse splenocytes and may be used at ≤0.03 µg/test (in 100 µL volume). Optimal titration is recommended for best performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) is a tandem dye emitting at 732 nm, optimized for cytometers with a 355 nm UV laser. Protect from light to prevent photo-induced oxidation.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific interactions.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (00-8222)
• 1-step Fix/Lyse Solution (00-5333)

BUV737 is compatible with short-term methanol fixation but should not be stored in methanol-containing buffers for over 1 hour.

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

MHC class II molecules are transmembrane glycoproteins expressed on antigen-presenting cells (macrophages, dendritic cells, B cells). They bind endocytosed exogenous antigens and present them to T cells. The antigen-binding groove between alpha and beta chains is open at both ends and accommodates 15–24 amino acid peptides.

HLA and MHC antibodies are crucial in Immunopeptidomics, aiding in neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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