Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: ≤ 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700, Plus 594
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and serves as a marker for determining the growth fraction of cell populations.

SolA15 also reacts with human, non-human primate, and canine Ki-67. It is reported for use in intracellular staining followed by flow cytometric analysis.

Recommended use:

• Tested with Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00)
• ≤ 0.25 µg per test (100 µL final volume)
• Cell number range: 10⁵–10⁸ cells/test
• Carefully titrate for optimal performance

Fluorochrome information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm, compatible with UV (355 nm) laser-equipped cytometers.

Staining buffer recommendations:
Use Super Bright Complete Staining Buffer (Product #SB-4401) or Brilliant Stain Buffer (Product #00-4409-75) to minimize polymer dye interactions.

Light sensitivity:
Protect vial and samples from light; the dye is sensitive to photo-induced oxidation.

Fixation compatibility:
Samples can be stored up to 3 days at 4°C in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) with minimal brightness loss.
BUV737 is compatible with short-term methanol fixation (<1 hour).

Excitation/Emission:

• Excitation: 355 nm
• Emission: 732 nm
• Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin regions and shows cell cycle-dependent localization. Ki-67 serves as a proliferation marker, absent in G0 cells and increasing through mitosis.

In neoplastic tissues, the Ki-67 labeling index correlates with tumor grade and prognosis. It is widely used as a neuronal and proliferation marker in research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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