Thermo Fisher Scientific Human MELK, GST Tag Recombinant Protein

Thermo Fisher Scientific Human MELK, GST Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution

• Assay-dependent

Product Specifications

Product Specific Information

• For maximum recovery, please spin prior to use.
• Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use original packaging until exhausted.
• For larger sizes, aliquot preparation can reduce freeze/thaws; aliquots below 20 µL are not recommended.
• Never store kinase diluted.
• Properly stored at -80°C, product guaranteed for 6 months from date of purchase.
• Protein Form: Catalytic

Target Information

MELK is a serine/threonine kinase related to the AMPK family, linked to the cell cycle and overexpressed in various cancers. It phosphorylates substrates including BCL2L14, CDC25B, MAP3K5/ASK1, and ZNF622. MELK acts as an activator of apoptosis by phosphorylating MAP3K5/ASK1 and regulates cell cycle progression through CDC25B phosphorylation and localization to centrosome and spindle poles. It plays a key role in cell proliferation and carcinogenesis, essential for embryonic and postnatal neural progenitor proliferation. MELK also inhibits spliceosome assembly during mitosis via ZNF622 phosphorylation, contributing to nuclear redirection and possibly affecting mammary carcinogenesis by modulating BCL2L14 activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.