Thermo Fisher Scientific CD31 Monoclonal Antibody (CO.3E1D4)

Applications

Flow Cytometry (Flow)

• Tested Dilution: 1:10–1:25

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent

Product Specifications

Additional Formats

• FITC 형식 보기
• 맞춤 결합 요청

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Mouse anti-Sheep CD31 antibody, clone CO.3E1D4 recognizes ovine CD31 (PECAM-1).
Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling through dual immunoreceptor tyrosine-based inhibitory motifs (ITIM).
Upon phosphorylation by associated kinases, these motifs provide docking sites for protein-tyrosine phosphatases.
CD31 is ubiquitously expressed within the vascular compartment, mainly at junctions between adjacent cells.
The N-terminal Ig-like domain is responsible for homophilic binding, playing a key role in cell-cell interactions.
CD31 functions in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immunoreceptor signaling regulation, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis, and thrombosis.
It is one of the key regulatory molecules in the vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.