Thermo Fisher Scientific EED Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

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Immunohistochemistry (IHC)

20 µg/mL

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Immunocytochemistry (ICC/IF)

20 µg/mL

-

ChIP assay (ChIP)

2.5 µg/10^6 cells

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An 18 amino acid synthetic peptide near the amino terminus of human EED if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EED,uniProtId:O75530-1,ncbiNodeId:9606,antigenRange:1,antigenLength:441,antigenImageFileName:PA5-34430_EED_O75530-1_Rabbit.svg,antigenImageFileNamePDP:PA5-34430_EED_O75530-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2551782

Product Specific Information

A suggested positive control is human heart tissue lysate.

PA5-34430 can be used with blocking peptide PEP-1472.

Target Information

The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin Beta-7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.